This iconic reaction comes from , specifically the Season 5 episode titled " Frame Toby " . In the scene, Michael Scott (played by Steve Carell) discovers that his HR nemesis, Toby Flenderson , has unexpectedly returned from Costa Rica and is back at his desk.

Watch the original scene where Michael Scott realizes his worst nightmare has returned to the office: "No God, Please No! Noooooooo!" - The Office [MEME ORIGIN] Prime Video Nederland YouTube• Nov 7, 2020

The moment has since become a legendary meme and GIF used to express extreme disbelief, horror, or frustration. According to the Office Ladies podcast, the sheer intensity of the scream was partially improvised by Carell.