Imaging — Flow Cytometry: Methods And Protocols

: Systems like the ImageStream use Charge-Coupled Device (CCD) cameras with Time Delay Integration (TDI) to track cells in flow, preventing motion blur and enabling the detection of dim markers.

: If internal markers (e.g., nuclear proteins) are the target, cells are fixed with formaldehyde and permeabilized with detergents like Triton X-100. Imaging Flow Cytometry: Methods and Protocols

: It allows for the quantification of intracellular features, such as the translocation of signals into the nucleus or the internalization of particles. Key Protocol Steps : Systems like the ImageStream use Charge-Coupled Device